Leverage uBriGene’s platform-based adenovirus packaging service with access to extensive skills in adenovirus manufacturing, QC testing, and regulatory support.
uBriGene utilizes a well-established adenovirus packaging process, enabling us to offer adenovirus packaging for discovery and preclinical studies, as well as GMP adenovirus for clinical use. Our comprehensive services include plasmid construction, seed virus packaging, and large-scale adenovirus manufacturing.
| Types of Services | Description | Estimated Timeline | Pricing |
|---|---|---|---|
| Research Grade Adenovirus Packaging | Adenovirus seed virus packaging and adenovirus expansion for discovery and preclinical studies | 2-3 Weeks | |
| GMP Adenovirus Manufacturing | Adenovirus GMP manufacturing for clinical applications | Varies | Please Inquire |
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Let’s leverage uBriGene’s extensive expertise in adenovirus packaging, including large-scale amplification and purification capabilities.
Streamlined AAV packaging processes
Ultracentrifugation or chromatographic purification
Adenovirus packaging begins with adenovirus plasmid construction, followed by virus packaging inside HEK293 cells after plasmid transfection. The adenovirus seed stock is then purified by ultracentrifugation or chromatography.
After the adenovirus seed viruses pass QC release, we can support master virus banking, further amplification and purification for large-scale adenovirus productions.
Adenovirus manufacturing/expansion involves virus amplification and purification. Host cells are transduced with seed viruses and expanded to the appropriate scale. After cell lysis and clarification, adenoviruses are purified using density gradient ultracentrifugation, anion exchange (AEX), or size exclusion chromatography. The final adenoviruses are formulated via tangential flow filtration (TFF) and sterilized using 0.22 µm filters.
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uBriGene provides AAV packaging services using both adherent HEK293 cells and our proprietary 293XS suspension cell production systems. The advantages of the suspension cell production system include being serum-free and easily scalable for preclinical and GMP AAV production.
Fig. 1. Adenovirus (AdV) elution profile after AEX chromatographic purification
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Wild-type adenovirus is a non-enveloped virus with a double-stranded DNA genome. Wild-type adenoviruses are replication-competent and can cause mild cold or flu-like symptoms. Recombinant adenovirus is engineered to be replication-deficient.
Recombinant adenovirus requires handling under Biosafety Level 2 (BSL-2). The recombinant adenovirus itself does not cause illness, but the function of the inserted genes should be carefully considered. Wild-type adenovirus generally does not cause serious illness, but it can cause mild cold or flu-like symptoms in healthy individuals.
Adenovirus is used in adenovirus-based vaccines for infectious pathogens, oncolytic virus therapy, gene editing, and RNAi therapy.
1. High packaging capacity, up to 7.5 kb, suitable for large genes.
2. High transduction efficiency, achieving nearly 100% transduction.
3. Broad cell spectrum—adenovirus vectors can infect not only common cell lines but also more challenging cells, such as suspension and primary cells.
Our adenovirus packaging service includes plasmid construction, virus packaging in HEK293 cells, and purification using ultracentrifugation or chromatography. We also provide plasmid preparation and sequencing confirmation to ensure quality.
The typical turnaround time for adenovirus packaging is 2-4 weeks, depending on the complexity of the project and scale required.
We offer various production scales ranging from small-scale research-grade production, large-scale preclinical batches, and GMP adenovirus production.
We use multiple quality control methods, including sequencing, titer determination, and sterility testing, to ensure the adenoviruses are free from contaminants and meet your specifications.
Discover the difference our AAV packaging services can make in your research. Get high-quality, high-yield AAV vectors with our state-of-the-art facilities and support.
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Established Processes for high quality and yield
R&D grade & GMP Adenovirus productions
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Ask the plasmid DNA expert! With over 300 GMP batches in our track record and our cost-effective platform technology, we can help accelerate your clinical programs.
