uBriGene’s innovative sgRNA in vitro transcription (IVT sgRNA) delivers high-efficiency, high-fidelity sgRNA with a scalable, cost-effective process for clinic translation.
uBriGene’s proprietary IVT sgRNA production has overcome the challenges of IVT-generated guide RNA, delivering higher gene editing efficiency without cytotoxicity.
The IVT sgRNA method has been optimized for scalability in a GMP environment, resulting in unprecedented cost- and time-savings.
| Types of Services | Description | Scale | Estimated Timeline | Pricing |
|---|---|---|---|---|
| Research Grade IVT sgRNA Production | sgRNA in vitro transcription production services for discovery and preclinical studies | µg to mg | 2-3 Weeks | |
| GMP IVT sgRNA Manufacturing | sgRNA IVT manufacturing meeting GMP requirements | mg to grams | 3-5 Weeks | Request a Quote |
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With our high productive RNA manufacturing platform and RNA-LNP formulation technologies, we can accelerate your RNA therapeutic and vaccine programs.
With our high productive RNA manufacturing platform and RNA-LNP formulation technologies, we can accelerate your RNA therapeutic and vaccine programs.
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UBriGene has developed a simple, rapid, and scalable IVT-based sgRNA production method that consistently produces IVT sgRNA of exceptional quality, while avoiding the environmental impact of chemical synthesis approaches.
The IVT sgRNA production process is more scalable and cost-effective compared to chemical-synthesis methods.
IVT sgRNA is safer.
No chemical reagents are used in in vitro transcription production of IVT sgRNA
IVT sgRNA is more accurate.
sgRNA-IVT preparation process uses high-quality templates and high-fidelity polymerase, while chemical synthesis of long RNA tends to have base mutations.
IVT sgRNA is more stable.
IVT sgRNA is modified at the 3' end of the sgRNA with an RNA aptamer sequence to enhance its stability.
Higher gene editing efficiency.
Gene editing tests show IVT sgRNA results in higher gene-editing efficiency compared to chemically synthesized sgRNA using RNP complexes with uBriGene’s Cas9 protein.
Gene editing efficiency was tested using IVT sgRNA and chemical-synthesized sgRNA in two separate gene editing projects.
uBriGene offers a comprehensive set of CRISPR gene editing tools for genetic engineering to support your cell and gene therapy programs, sgRNA IVT, Cas proteins, Cas mRNA, and donor DNA.
Our sgRNA is produced using our innovative in vitro transcription method, combined with Cas proteins (as RNP) or Cas mRNA (as LNP) for efficient gene editing. If donor DNA is required, we can also produce more stable closed-end linear double-stranded DNA or AAV vectors containing the donor DNA.
Diagram of CRISPR gene editing tools from uBriGene
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uBriGene guarantees a 7-business day turnaround time from order placement to sgRNA shipment.
We can produce IVT sgRNA in quantities ranging from micrograms to grams. The production process for both R&D-grade and GMP-grade sgRNA is the same, with GMP production following stricter regulatory standards.
Yes, the uBriGene team can assist with the design and manufacturing of reagents needed for KI studies.
Yes, uBriGene experts are available to assist clients with LNP formulation development studies.
We can produce IVT sgRNA of 250 nt and longer. Unlike chemical synthesis, the in vitro transcription method has minimal limitations on the length of the guide RNA.
Tap into our mRNA, circRNA, and saRNA manufacturing and LNP formulation expertise, focus your time on discovery research!
Scalable and cost-effective process
Higher gene editing efficiency
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