Super active Ubrinuclease™ pan endonuclease, purified in compliance with regulatory standards, exhibits broad nuclease activity for all types of nucleic acids.
The Ubrinuclease™ pan endonuclease effectively digests any form of DNA and RNA (double-stranded, single-stranded, linear, circular, native, or denatured) into 3-5 bases 5'-monophosphate oligonucleotides under a wide range of conditions.
uBriGene has assisted our clients in obtaining CAR-T IND clearance using LVV manufactured with these lentivirus packaging plasmids.
High purity & high pan endonuclease activity
Free of Animal derived materials, fully compliant with regulatory standards
Ideal for removing DNA and RNA in the production of biologics
Tag-free or His/ Strep-Tag
The various Rep/Cap plasmids and helper plasmids have been tested for high quality and high yield in GMP AAV production.
We have assisted our clients in obtaining IND clearance with AAV produced using these AAV helper plasmids.
| Product Name | Quality Grade | Catalog Number | Size | Price |
|---|---|---|---|---|
| Ubrinucelase™ Pan endonuclease | GMP grade | UbNuC-G100 | 100KU | Please Inquire |
| UbNuC-G500 | 500KU | |||
| UbNuC-G1000 | 1MU |
GMP grade pan endonuclease that works with any DNA and RNA.
Request a Quote
Ubrinucelase™ pan endonuclease (GMP grade) can be used for discovery research, and as well as GMP manufacturing of cell and gene therapies, vaccines, and other biologics. The GMP-grade Ubrinucelase™ pan endonuclease products are manufactured in compliance with the highest regulatory standards.
Below are a few examples of applications that the pan endonuclease can used:
Protein production to reduce viscosity, increasing protein yield.
Viral vector manufacturing to remove nucleic acids on the surface of viral particles, decreasing unwanted residual DNA.
Sample preparation, including ELISA, chromatography, 2D electrophoresis, and blotting, improving resolution and recovery rates.
Vaccine, polysaccharides, and other biologics to remove residual nucleic acids.
Preventing PBMC clumping in cell therapy and vaccine research
| Source | S. marcescens |
|---|---|
| Expression Host | E. coli |
| Molecular Weight | ～30KD |
| Isoelectric Point | 6.39/6.85/7.1 |
| Appearance | Clear |
| PH | 7.0-9.0 |
| Tag | Tag-free/ His-tag/ Strep-tag |
| Purity | ≥99%（SDS-PAGE/SEC-HPLC） |
| Specific Activity (calc. on protein) | ≥ 1.0×106 U/mg |
| Enzyme Activity | ≥ 250 U/μL |
| Activity Definition | One unit (U) is defined as the amount of enzyme required to change the absorbance at 260 nm by 1.0 (equivalent to complete digestion of 37 μg of salmon sperm DNA into oligonucleotides) in a 2.625 mL reaction system at 37°C, pH 8.0, within 30 minutes. |
| Sterility | Negative |
| Mycoplasma | Negative |
| Microbial Count | ＜1cfu/mL |
| Endotoxin | ＜0.25EU/KU |
| HCP | ＜10μg/mg |
| Heavy Metal Residuals | ≤10ppm |
| Protease Activity | Negative |
| Buffer | 20 mM Tris-HCl pH8.0，2 mM MgCl2，20 mM NaCl，50% glycerin |
| Optimal Temperature & pH | 37℃，8.0 |
Shipping on ice; stored at -20°C for long-term storage, with a shelf life of 2 years.
SDS-PAGE and SEC-HPLC analysis confirm Ubrinucelase™ Pan Nuclease has a purity above 99%.
Pure Ubrinuclease™ Pan Endonuclease analyzed by SDS-PAGE (left) and SEC-HPLC (right).
Ubrinuclease™ shows superior endonuclease activity compared to competitor products and is more tolerant of varying salt concentrations (0–0.5M NaCl, 30 min). This adaptability improves its efficiency in diverse biologics purification processes.
Notes for endonuclease:
EDTA, urea, SDS, and guanidine hydrochloride can inhibit or inactivate the enzyme.
Repeated freeze-thaw cycles affect the enzymatic activity. Small aliquots are recommended for storage; do not store at -80°C.
For high-salt, acidic, or basic solutions, or those with high concentrations of detergents or denaturants, increase enzyme dosage or extend incubation time to maximize enzyme activity.
Pure UbrinucleaseTM Pan Endonuclease analyzed by SDS-PAGE (left) and SEC-HPLC (right).
Due to its pan endonuclease activity, the Ubrinuclease™ Pan Endonuclease is ideal in removing unwanted DNA and RNA in assays and biologics productions, lowering the residuals to the safety level. Its broad range of activity makes it suitable for various cell and gene therapy workflows and biologics manufacturing.
Yes, our pan endonuclease can remove any form of DNA and RNA, any form of DNA and RNA (double-stranded, single-stranded, linear, circular, native, or denatured) into 3-5 bases 5'-monophosphate oligonucleotides.
Ubrinuclease™ demonstrates superior activity compared to competitor endonucleases with high tolerance for varying salt concentrations (0–0.5M NaCl), enhancing efficiency across diverse biologics purification processes.
Yes, Ubrinuclease™ is designed for GMP-compliant production environments, ensuring quality and regulatory compliance for use in the manufacture of therapeutic biologics and gene therapy products.
Extensive expertise with a track record of successfully releasing over 60 GMP batches of AAV.
Digest on any DNA and RNA
Manufactured according to GMP requirement
Sit aliquam elit vitae bibendum lectus blandit dictum. Nisi porta elementum sed diam eleifend pellentesque. Quis aenean quam.
Request a QuoteOptional caption section: This caption text can be used under any image or content block as-needed. Use cases are figure legends, citations, image descriptions
Ask the plasmid DNA expert! With over 300 GMP batches in our track record and our cost-effective platform technology, we can help accelerate your clinical programs.
