High-efficiency CRISPR gene editing products, including Cas proteins and RNAs, ensure seamless, effective gene editing for your cell and gene therapies.
uBriGene offers CRISPR gene editing products, including Cas proteins and mRNAs such as spCas9, Cas12a, Cas13a, and Cas14.
These gene editing proteins and mRNAs are manufactured under GMP standards and fully tested for quality and gene editing efficiency.
A comprehensive range of gene editing products, including spCas9, Cas12a, Cas13a, and Cas14.
Traceable and complete documentation.
MP-grade products to support gene modification in clinical cell and gene therapies.
Full-service solutions, including RNP and LNP formulation with your guide RNA.
The various Rep/Cap plasmids and helper plasmids have been tested for high quality and high yield in GMP AAV production.
We have assisted our clients in obtaining IND clearance with AAV produced using these AAV helper plasmids.
| Product Name | Cat# | Description | R&D Grade | GMP Grade | Price |
|---|---|---|---|---|---|
| Ubri-Cas9 | 1308Ubri-Cas9 | spCas9 Protein, high quality and tested for efficient gene editing | Yes | Yes | |
| Ubri-Cas12a | 1308Ubri-Cas12a | Cas12a Protein, high quality and tested for efficient gene editing | Yes | Yes | |
| Ubri-Cas13a | 1308Ubri-Cas13a | Cas13a Protein, high quality and tested for efficient gene editing | Yes | Yes | |
| Ubri-Cas14 | 1308Ubri-Cas14 | Cas14 Protein, high quality and tested for efficient gene editing | Yes | Yes | Please Inquire |
| spCas9-mRNA | spCas9-mRNA | Cas9 mRNA, ultra-pure, tested for high gene editing efficiency | Yes | Yes | |
| Cas12a-mRNA | Cas12a-mRNA | Cas12a mRNA, ultra-pure, tested for high gene editing efficiency | Yes | Yes | |
| Cas13a-mRNA | Cas13a-mRNA | Cas13a mRNA, ultra-pure, tested for high gene editing efficiency | Yes | Yes | |
| Cas14-mRNA | Cas14-mRNA | Cas14 mRNA, ultra-pure, tested for high gene editing efficiency | Yes | Yes |
Cas proteins & Cas mRNAs
Manufactured under GMP standards
Fully tested for high gene editing efficiency
RNP and LNP formulation services
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uBriGene has developed sgRNA in vitro transcription technology, which is more scalable and cost-effective than chemical synthesis.
Extensive plasmid production experience
100+ GMP plasmid projects annually
uBriGene offers comprehensive CRISPR gene editing tools for both discovery research and clinical programs. Our ready-to-use Cas proteins and mRNAs, combined with our groundbreaking sgRNA in vitro transcription technology, support all your gene editing needs.
uBriGene’s lentivirus packaging plasmids were used for lentiviral vector production with uBriGene’s proprietary LVV suspension cell bank, 293TH. Transduction titer was measured using flow cytometry after transducing Jurkat cells.
CRISPR Cas proteins are recombinant proteins purified from E. coli using a series of chromatography methods. The untagged Cas proteins are concentrated by TFF and sterile filled into vials, ready for gene editing in clinical therapies.
Fig. 1. Diagram of CRISPR Cas protein purification process.
Ultra-pure Cas proteins are produced using our optimized protein purification processes. The following data shows the elution profile of Cas9 using a chromatographic method.
Fig. 2. Cas9 elution profile during chromatographic purification.
The activity of Cas9 proteins was analyzed using an in vitro assay by cutting linear DNA in the presence of sequence-specific guide RNA. Cas9 proteins from uBriGene demonstrated higher activity than those from both competitors.
Fig. 3. Cas9 proteins from uBriGene and two competitors were tested for guide RNA-mediated, DNA sequence-specific cutting in an in vitro assay.
CRISPR Cas proteins are recombinant proteins purified from E. coli using a series of chromatography methods. The untagged Cas proteins are concentrated by TFF and sterile filled into vials, ready for gene editing in clinical therapies.
Our off-the-shelf lentivirus packaging plasmids are manufactured and released in strict compliance with the highest regulatory standards. A certificate of analysis will be provided with each of the GMP grade lentiviral packaging plasmids.
| Test Items | Method | |
|---|---|---|
| Appearance | Visual inspection | |
| pH | pH measurement | |
| Concentration | UV, Spectrophotometry | |
| Identity | Restriction analysis | Gel electrophoresis |
| Target sequence analysis* | Sanger sequencing | |
| Purity | A260/A280 | UV, Spectrophotometry |
| Supercoiled plasmid ratio | HPLC | |
| Residuals | Residual E. Coli DNA | qPCR |
| Residual E. Coli RNA | Gel electrophoresis/HPLC | |
| Residual E. Coli protein | ELISA | |
| Safety | Endotoxin | Gel-clot assay |
| Sterility | Membrane filtration method | |
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Yes, uBriGene’s Cas proteins and mRNAs have been manufactured in compliance with regulatory standards, and have been fully QC released. The products have full traceable documents.
Yes, we have tested gene editing efficiency using Cas9 mRNA and guide RNA formulated into LNPs. uBriGene also supports RNA-LNP formulation studies with our proprietary microfluidic technology.
Cas proteins are CRISPR-associated proteins that play a critical role in the CRISPR gene editing system. Different Cas systems may be used in gene editing, RNA editing, and diagnostics.
uBriGene offers a range of Cas proteins, including spCas9, Cas12a, Cas13a, and Cas14. These proteins are designed for various applications in gene editing, allowing for flexibility in research and clinical use.
Our Cas proteins are produced as recombinant proteins purified from E. coli using optimized protein purification processes. This ensures high purity and activity, suitable for various applications.
All uBriGene Cas proteins and mRNAs are manufactured under GMP standards and undergo rigorous testing for quality and gene editing efficiency. We provide traceable documentation to ensure compliance and reliability.
You can order our products by phone call or email, 800 663 2528, contact@ubrigene.com.
Yes, uBriGene provides complete solutions, including assistance with RNP (ribonucleoprotein) and LNP (lipid nanoparticle) formulation using your guide RNA. Our team is here to support you throughout the process. If you need sgRNA production, our innovative sgRNA in vitro transcription is the best choice, as it is more scalable and cost effective for large scale sgRNA productions.
Cas proteins and mRNAs are used in various applications, including basic research, gene therapy, and precision medicine. They enable targeted gene editing, gene knockouts, and more, across multiple fields of study.
Extensive expertise with a track record of successfully releasing over 60 GMP batches of AAV.
Manufactured under GMP standards
Fully tested for high gene editing efficiency
RNP and LNP formulation services
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Ask the plasmid DNA expert! With over 300 GMP batches in our track record and our cost-effective platform technology, we can help accelerate your clinical programs.
