A ready-to-use, non-integrating reprogramming product featuring an mRNA-LNP cocktail for efficient generation of induced pluripotent stem cells (iPSCs).
The iPSC Reprogramming Cocktail is a simple LNP composed of mRNAs for five reprogramming factors. It efficiently reprograms human skin fibroblasts and PBMC to iPSCs with low toxicity compared to other RNA kits.
Reprogramming with mRNA, no risk of gene integration
Convenient, Ready-to-use LNP mix, can be directly added to cells, eliminating the need for transfection reagents or viral manipulation
Low cytotoxicity, high iPSC reprogramming efficiency
System flexibility: suitable for feeder or feeder-free conditions
The various Rep/Cap plasmids and helper plasmids have been tested for high quality and high yield in GMP AAV production.
We have assisted our clients in obtaining IND clearance with AAV produced using these AAV helper plasmids.
| Product Name | Cat# | Description | R&D Grade | GMP Grade | Product Size | Price |
|---|---|---|---|---|---|---|
| iPSC-reprogram-mRNA-S | iPSC-RPM-RNA-S | Efficient iPSC reprograming cocktail mRNA, 0.1ug/uL, trial size | Yes | No | 2x60uL (1x iPSC reprogramming) | |
| iPSC-reprogram-mRNA | iPSC-RPM-RNA | Efficient iPSC reprograming cocktail mRNA, 0.1ug/uL | Yes | Yes | 8x60uL (5x iPSC reprogramming) | |
| iPSC-reprogram-mRNA -LNP-S | iPSC-RPM-RNA-LNP-S | Efficient iPSC reprograming mRNA-LNP cocktail, can be directly added to cells, 0.1 ug/uL, trail size, | Yes | No | 2x60uL (1x iPSC reprogramming) | Please Inquire |
| iPSC-reprogram-mRNA -LNP | iPSC-RPM-RNA-LNP | Efficient iPSC reprograming mRNA-LNP cocktail, can be directly added to cells | Yes | Yes | 8x60uL (5x iPSC reprogramming) | |
| iPSC-Cell-Banks | iPSC-BANK | iPSC cell banks generated using uBriGene’s reprogramming mRNA-LNP mix. | Yes | Yes | 1mL/vial | |
| iPSC-Cell Culture-Media | iPSC-MEDIA | Media for iPSC cell culturing | Yes | Yes | 500 mL/bottle |
GMP grade iPSC reprogramming mRNA & mRNA-LNP cocktail
iPSC Cell Banks
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Extensive iPSC generation and banking experience with access to essential raw materials, including iPSC reprogramming mRNA-LNP mix and CRISPR gene editing tools.
Extensive plasmid production experience
100+ GMP plasmid projects annually
Somatic cells were seeded into feeder or feeder-free plates on day 0, and treated with the iPSC Reprogramming mRNA-LNP Cocktail on day 1. After eight consecutive treatments with the iPSC Reprogramming Cocktail, the cells were maintained in a reprogramming medium for the remainder of the induction phase until iPSC cell colonies emerged. Typically, by day 15, the iPSC colonies are large enough to be selected and expanded.
Fig. 1. iPSC reprogramming diagram using uBriGene’s reprogramming mRNA-LNP cocktail mix.
By day 4, cell morphology changed significantly. Deformable cells increased by day 6, and aggregated cells appeared by day 8 of reprogramming. iPSC-like cells emerged on day 11, and typical iPSC colonies formed by day 13.
Fig. 2. Cell morphology after adding iPSC reprogramming mRNA-LNP.
Human skin fibroblasts were reprogrammed into iPSCs using uBriGene’s iPSC Reprogramming mRNA-LNP Cocktail, and the colonies were identified with AP staining on day 18. The results showed that iPSC colonies were positive for AP staining, with reprogramming efficiency reaching as high as 10%.
Fig. 3. iPSC identification using Alkaline phosphatase staining after the iPSC reprogramming process with mRNA-LNP cocktail.
Flow cytometry analysis showed that iPSCs cell banks generated by iPSC Reprogramming mRNA-LNP Cocktail express undifferentiated cell markers (SSEA4, TRA-1-81, Sox2 and Nanog).
Fig. 4. Detection of pluripotency identification markers for uBriGene’s iPSC cell bank, including SSEA4, TRA-1-81, Sox2, and Nanog, using flow cytometry.
iPSC cell banks generated using uBriGene’s reprogramming mRNA-LNP cocktail are tested for pluripotency. Embryoid bodies are differentiated from the iPSCs and specific cell lineage cell markers are detected via immunofluorescence staining.
Fig. 5. iPSC cells were differentiated into embryoid bodies to demonstrate the pluripotency of the iPSCs generated using our iPSC reprogramming mRNA-LNP cocktail. Immunostaining was performed for Nestin, MAP2, AFP, and SMA,
This simplified iPSC protocol is used for iPSC generation from fibroblast somatic cells using our reprogramming mRNA-LNP cocktail.
D0: Plate fibroblasts onto a 6-well plate pre-coated with BioLaminin in fibroblast culture media.
D1-8: Change media to ReproTeSR containing B18R. Administer 1 μg of the mRNA-LNP cocktail (10 μL of uBriGene’s mRNA-LNP mix) per well daily for 8 consecutive days, ensuring the medium is changed every 18–24 hours after adding the LNP.
D9: Switch to ReproTeSR media without B18R; split cells if needed.
D10-15: Change media daily with ReproTeSR, monitoring for iPSC clones.
D16-18: Pick and expand iPSC colonies. Confirm iPSC formation by Alkaline Phosphatase (AP) staining and validate pluripotency with markers such as SSEA4, TRA-1-81, SOX2, and NANOG.
For the subsequent maintenance and expansion of the iPSCs, culture them in uBriGene’s iPSC culture medium on laminin-coated plates. By Days 27–32, the iPSCs can be cryopreserved in a freezing medium for seed banking.
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uBriGene’s iPSC Reprogramming mRNA-LNP cocktail is a ready-to-use lipid nanoparticle formulation containing mRNAs for five reprogramming factors. It is designed to efficiently reprogram somatic cells, like human fibroblasts, into iPSCs without altering the cell genome.
The mRNA-LNP cocktail delivers reprogramming mRNAs into somatic cells, triggering them to convert into induced pluripotent stem cells (iPSCs). This non-integrating method reduces the risk of permanent changes to the genome.
mRNA-LNP reprogramming cocktail avoids the risk of gene integration, offers high efficiency, and has low cytotoxicity. It also simplifies the process since no transfection reagents or viral manipulation are required.
The reprogramming process usually takes about 15–18 days. iPSC colonies typically emerge around day 13, and are large enough for selection and expansion by day 15.
Yes, iPSCs generated using uBriGene’s mRNA-LNP cocktail are pluripotent. They express key pluripotency markers such as SSEA4, TRA-1-81, Sox2, and Nanog.
The iPSC reprogramming mRNA-LNP cocktail has been optimized for use with human fibroblasts and PBMC, but can be tested on other somatic cells as well, depending on the research needs.
iPSC cell banks are tested for the expression of undifferentiated cell markers (SSEA4, TRA-1-81, Sox2, and Nanog) via flow cytometry to ensure high-quality iPSC generation.
uBriGene’s iPSC products, including cell banks, are manufactured in compliance with GMP standards, making them suitable for preclinical and clinical applications.
We offer off-the-shelf GMP-grade Cas proteins and mRNAs. Additionally, uBriGene has developed an innovative in vitro transcription technology for sgRNA, providing higher scalability and cost-effectiveness for large-scale sgRNA production.
Yes, we follow the guidelines of 21 CFR 1271, have detailed governing documentation, Trial Master files for donors.
Extensive expertise with a track record of successfully releasing over 60 GMP batches of AAV.
GMP grade iPSC reprogramming mRNA & mRNA-LNP cocktail
iPSC Cell Banks for clinical use
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