Lentivirus purified through an optimized manufacturing process, highly potent with transduction units defined by flow cytometry.
uBriGene’s lentiviral products are produced with extensive expertise in lentivirus production and are supported by a comprehensive suite of quality control testing.
Each batch includes actual transduction titers measured via flow cytometry to ensure high-quality performance.
Purified and high-quality lentivirus
Transduction titer determined using flow cytometry
Large scale and GMP lentivirus production capabilities
Custom lentivirus production available for your gene of interest
The various Rep/Cap plasmids and helper plasmids have been tested for high quality and high yield in GMP AAV production.
We have assisted our clients in obtaining IND clearance with AAV produced using these AAV helper plasmids.
| Products | Product Name | Catalog Number | Description | Volume | Price |
|---|---|---|---|---|---|
| GFP Lentivirus | LV-CMV-C-GFP-E7 | 1305LV-CMV-C-GFP-E7 | Lentivirus, expressing CMV-GFP, Neomycin resistance, 10^7 TU/mL |
200 uL | |
| LV-CMV-C-GFP-E8 | 1305LV-CMV-C-GFP-E8 | Lentivirus, expressing CMV-GFP, Neomycin resistance, 10^8 TU/mL |
200 uL | ||
| LV-CMV-C-GFP-E9 | 1305LV-CMV-C-GFP-E9 | Lentivirus, expressing CMV-GFP, Neomycin resistance, 10^9 TU/mL |
100 uL | ||
| Luciferase-mWasabi Lentivirus | LV-CMV-Luciferase-mWasabi-E7 | 1305LV-CMV-Luciferase-mWasabi-E7 | Lentivirus, expressing CMV-Luciferase-mWasabi, blasticidin resistance, 10^7 TU/mL |
200 uL | |
| LV-CMV-Luciferase-mWasabi-E8 | 1305LV-CMV-Luciferase-mWasabi-E8 | Lentivirus, expressing CMV-GFP, blasticidin resistance, 10^8 TU/mL |
200 uL | ||
| LV-CMV-Luciferase-mWasabi-E9 | 1305LV-CMV-Luciferase-mWasabi-E9 | Lentivirus, expressing CMV-GFP, blasticidin resistance, 10^9 TU/mL |
100 uL | Please Inquire | |
| Luciferase Lentivirus | LV-CMV-Luciferase-E7 | 1305LV-CMV-Luciferase-E7 | Lentivirus, expressing CMV-Luciferase, 10^7 TU/mL | 200 uL | |
| LV-CMV-Luciferase-E8 | 1305LV-CMV-Luciferase-E8 | Lentivirus, expressing CMV-Luciferase, 10^8 TU/mL | 200 uL | ||
| LV-CMV-Luciferase-E9 | 1305LV-CMV-Luciferase-E9 | Lentivirus, expressing CMV-Luciferase, 10^9 TU/mL | 100 uL | ||
| Raji-Luc cells | Raji-LUC | Raji-LUC | Raja cells stably expressed with luciferase and mWasabi, blasticidin resistance, single cell colonies isolated using flow cytometry. |
12E6 cells/mL, 1mL |
Note: If different titers and requirements for the lentivirus are needed, uBriGene provides custom lentivirus production. Contact us.
Request a quoteInfectious titer measured using flow cytometry
Best for in vivo animal studies and in vitro cell culture
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As an experienced lentivirus CDMO provider, uBriGene supports your lentivirus packaging discovery and preclinical needs with scalable and optimized production processes.
Extensive plasmid production experience
100+ GMP plasmid projects annually
uBriGene utilizes a third-generation, four-plasmid lentivirus packaging system—pGag/Pol, pRev, pVSV-G, and GOI—for enhanced safety.
Lentiviral packaging plasmids and the gene of interest (GOI) are transiently transfected into HEK293 cells or uBriGene’s proprietary suspension cells, 293TH, followed by lentivirus purification via ultracentrifugation or chromatography
Lentiviral particles, being enveloped viruses, tend to lose their infectivity easily. uBriGene has optimized its production process to ensure highly potent lentivirus. Infectious titers are measured using flow cytometry after cell transduction.
uBriGene’s lentivirus packaging plasmids were used for lentiviral vector production with uBriGene’s proprietary LVV suspension cell bank, 293TH. Transduction titer was measured using flow cytometry after transducing Jurkat cells.
HEK293 cells were transduced with GFP lentivirus. Fluorescent images taken 48 hours post transduction.
Raji-Luciferase stable cells are developed by transducing Raji cells with luciferase-mWasabi lentivirus, followed by single-cell isolation using flow cytometry. These Raji-Luciferase cells are commonly used in CAR-T cell killing assays.
| Test Items | Method | |
|---|---|---|
| Appearance | Visual inspection | |
| pH | pH measurement | |
| Concentration | UV, Spectrophotometry | |
| Identity | Restriction analysis | Gel electrophoresis |
| Target sequence analysis* | Sanger sequencing | |
| Purity | A260/A280 | UV, Spectrophotometry |
| Supercoiled plasmid ratio | HPLC | |
| Residuals | Residual E. Coli DNA | qPCR |
| Residual E. Coli RNA | Gel electrophoresis/HPLC | |
| Residual E. Coli protein | ELISA | |
| Safety | Endotoxin | Gel-clot assay |
| Sterility | Membrane filtration method | |
Unveil the current manufacturing approaches for LVV and its deployment in cell therapies.
Learn about CAR-T production process & regulatory guidelines.
Learn about uBriGene’s RNA and LNP manufacturing capacities and testing.
Learn how uBriGene can support your discovery and clinical programs.
The lentiviral particles from uBriGene are pseudotyped lentiviruses using VSV-G as the envelope protein, enabling broad cell tropism. They can transduce most cell types.
uBriGene's lentiviruses are third-generation, replication-deficient particles with a biosafety level of BSL-2.
Functional titer assays are performed via cell culture-based transduction assays and measured by flow cytometry. uBriGene’s transduction titers provide an accurate reflection of the actual infectious titer.
The multiplicity of infection (MOI) represents the number of genomic or infectious particles per cell. Optimal MOI varies by cell line; it’s recommended to test a range of MOIs from 5 to 100 using positive lentiviral control particles with a fluorescent protein marker, such as uBriGene's GFP control lentivirus.
Lentivirus is popular due to its large packaging capacity and ability to stably integrate into the host genome for long-term gene expression. Most recombinant lentiviruses are pseudotyped with the VSV-G envelope protein, giving them broad cell tropism.
Lentiviruses are shipped on dry ice. For long-term storage, lentiviral particles should be aliquoted and stored at -80°C.
Extensive expertise with a track record of successfully releasing over 60 GMP batches of AAV.
Ready-to-use Lentiviruses
Custom lentivirus productions
Research and GMP lentivirus productions
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Ask the plasmid DNA expert! With over 300 GMP batches in our track record and our cost-effective platform technology, we can help accelerate your clinical programs.
